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r1000  (R&D Systems)


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    Structured Review

    R&D Systems r1000
    R1000, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 298 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/r1000/Rat+IL-10+Quantikine+ELISA+Kit/pm42013922-90-30-31
    Average 95 stars, based on 298 article reviews
    r1000 - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: New Insights into Hepatic and Intestinal Microcirculation and Pulmonary Inflammation in a Model of Septic Shock and Venovenous Extracorporeal Membrane Oxygenation in the Rat
    Article Snippet: .. TNF-α, IL-6, IL-10, CXCL2, and CXCL5 were measured by enzyme-linked immune sorbent assays according to the manufacturer’s instructions (ELISA kits R6000B, RTA00, and R1000, R&D System, Wiesbaden, Germany; ELISA kits ERCXCL2 and ERCXCL5, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Carotid body sensing of systemic inflammation contributes to recruitment of the splanchnic anti-inflammatory pathway.
    Article Snippet: .. Cytokine levels were measured in plasma and spleen samples using Quantikine ELISA kits according to the manufacturer’s instructions (TNF-α: catalog # RTA00, IL-6: catalog # R6000B, and IL-10: catalog # R1000, R&D Systems, Minneapolis, MN, USA). ..

    Article Title: Kaempferol prevents angiogenesis of rat intestinal microvascular endothelial cells induced by LPS and TNF-α via inhibiting VEGF/Akt/p38 signaling pathways and maintaining gut-vascular barrier integrity.
    Article Snippet: Kaempferol is a major flavonoid found in natural plant extracts; it shows great potential in anti-inflammatory and anti-cancer medicine.. However, the underlying mechanism of the protective action of kaempferol on the gutvascular barrier (GVB) and the active sites preventing intestinal micro-angiogenesis has not been reported.. The purpose of our study is to investigate the protective effect of kaempferol on the barrier damage induced by lipopolysaccharide (LPS) and tumor necrosis factor-alpha (TNF-α), and its mechanism of protective action on intestinal micro-angiogenesis.

    Article Title: A Novel Model of Venovenous Extracorporeal Membrane Oxygenation in Rats with Femoral Cannulation and Insights into Hemodynamic Changes
    Article Snippet: .. The cytokines tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 10 (IL-10) were measured using enzyme-linked immunosorbent assays performed according to the manufacturer’s instructions (ELISA kits R6000B, RTA00, and R1000, R&D Systems, Wiesbaden, Germany). ..

    Article Title: Zileuton alleviates acute cisplatin nephrotoxicity: Inhibition of lipoxygenase pathway favorably modulates the renal oxidative/inflammatory/caspase-3 axis.
    Article Snippet: Objective: The current study investigated for the first time the possible beneficial effect of zileuton, a selective 5lipoxygenase inhibitor (5-LOX), against cisplatin-induced acute renal failure.. Methodology: Male Sprague-Dawley rats (180–200 g) were administered cisplatin once (5mg/kg, i.p.) alone or combined with oral zileuton (10mg/kg, given twice; 1 h before and 12 h after cisplatin).. Results: Compared with control rats, acute cisplatin administration caused significant increases of BUN (33.76 ± 7.74 vs 61.88 ± 11.35mg/dl), serum creatinine (0.61 ± 0.21 vs 1.56 ± 0.28mg/dl), renal levels of MDA (6.40 ± 1.04 vs 20.52 ± 2.18 nmol/g tissue), NOx (3.45 ± 1.20 vs 17.70 ± 2.27 nmol/g tissue), TNF-α (6.71 ± 0.66 vs 23.71 ± 3.41 pg/g tissue), MPO (0.87 ± 0.09 vs 3.12 ± 0.41 U/mg tissue protein) and renal caspase-3 activity (2.81 ± 0.37 vs 12.70 ± 2.94 U/mg tissue protein).

    Article Title: Dynamic expression of the suppressor of cytokine signaling-3 and cytokines in the cerebral basilar artery of rats with subarachnoid hemorrhage, and the effect of acetylcholine.
    Article Snippet: Background There are complex interactions between acetylcholine (ACh), the suppressor of cytokine signaling-3 (SOCS3), and cytokines, however, little is known about their dynamic expression or their effects on cerebral vasospasm (CVS) after subarachnoid hemorrhage (SAH).. Therefore, we aimed to describe and clarify the dynamic expression of SOCS-3 and cytokines after SAH, as well as the relationships between the levels of SOCS-3, cytokines, and ACh.. Methods The rat model of single cisterna magna injection was used to mimic acute SAH.

    Clinical Proteomics:

    Article Title: Carotid body sensing of systemic inflammation contributes to recruitment of the splanchnic anti-inflammatory pathway.
    Article Snippet: .. Cytokine levels were measured in plasma and spleen samples using Quantikine ELISA kits according to the manufacturer’s instructions (TNF-α: catalog # RTA00, IL-6: catalog # R6000B, and IL-10: catalog # R1000, R&D Systems, Minneapolis, MN, USA). ..

    Article Title: New Insights into Hepatic and Intestinal Microcirculation and Pulmonary Inflammation in a Model of Septic Shock and Veno-Arterial Extracorporeal Membrane Oxygenation in the Rat
    Article Snippet: .. Systemic and pulmonary inflammation was assessed by measuring the concentrations of the cytokines TNFα, IL-6, IL-10, CXCL2, and CXCL5 in the plasma and BAL using enzyme-linked immune sorbent assays R6000B, RTA00, and R1000 from R&D System (Wiesbaden, Germany) and ERCXCL2 and ERCXCL5 from Thermo Fisher Scientific (Waltham, MA, USA) according to the manufacturer’s instructions; the probes were only thawed once. ..

    Article Title: New Insights into Hepatic and Intestinal Microcirculation and Pulmonary Inflammation in a Model of Septic Shock and Veno-Arterial Extracorporeal Membrane Oxygenation in the Rat.
    Article Snippet: .. Systemic and pulmonary inflammation was assessed by measuring the concentrations of the cytokines TNFα, IL-6, IL-10, CXCL2, and CXCL5 in the plasma and BAL using enzyme-linked immune sorbent assays R6000B, RTA00, and R1000 from R&D System (Wiesbaden, Germany) and ERCXCL2 and ERCXCL5 from Thermo Fisher Scientific (Waltham, MA, USA) according to the manufacturer’s instructions; the probes were only thawed once. ..

    Article Title: Dynamic expression of the suppressor of cytokine signaling-3 and cytokines in the cerebral basilar artery of rats with subarachnoid hemorrhage, and the effect of acetylcholine.
    Article Snippet: Background There are complex interactions between acetylcholine (ACh), the suppressor of cytokine signaling-3 (SOCS3), and cytokines, however, little is known about their dynamic expression or their effects on cerebral vasospasm (CVS) after subarachnoid hemorrhage (SAH).. Therefore, we aimed to describe and clarify the dynamic expression of SOCS-3 and cytokines after SAH, as well as the relationships between the levels of SOCS-3, cytokines, and ACh.. Methods The rat model of single cisterna magna injection was used to mimic acute SAH.



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    R&D Systems cd206
    SPION localize to CNS macrophage. SPION (visible as amber beads) were detected within Iba1 + , CD68 + , CD163 + , and <t>CD206</t> + perivascular, meningeal, and choroid plexus macrophages in the CNS. Macrophages were identified by hematoxylin and Eosin (H&E) staining and by immunohistochemistry (IHC) for Iba1, CD68, CD163, and CD206. Rows depict representative examples of perivascular, meningeal, and choroid plexus macrophages identified by anatomical location and IHC labeling. SPION are indicated by arrowheads in magnified images. Data are representative of N = 2 non-infected monkeys, 14 days post i.c. SPION inoculation
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    Image Search Results


    SPION localize to CNS macrophage. SPION (visible as amber beads) were detected within Iba1 + , CD68 + , CD163 + , and CD206 + perivascular, meningeal, and choroid plexus macrophages in the CNS. Macrophages were identified by hematoxylin and Eosin (H&E) staining and by immunohistochemistry (IHC) for Iba1, CD68, CD163, and CD206. Rows depict representative examples of perivascular, meningeal, and choroid plexus macrophages identified by anatomical location and IHC labeling. SPION are indicated by arrowheads in magnified images. Data are representative of N = 2 non-infected monkeys, 14 days post i.c. SPION inoculation

    Journal: Acta Neuropathologica Communications

    Article Title: SPION + macrophage traffic out of the central nervous system with SIV infection

    doi: 10.1186/s40478-025-02179-6

    Figure Lengend Snippet: SPION localize to CNS macrophage. SPION (visible as amber beads) were detected within Iba1 + , CD68 + , CD163 + , and CD206 + perivascular, meningeal, and choroid plexus macrophages in the CNS. Macrophages were identified by hematoxylin and Eosin (H&E) staining and by immunohistochemistry (IHC) for Iba1, CD68, CD163, and CD206. Rows depict representative examples of perivascular, meningeal, and choroid plexus macrophages identified by anatomical location and IHC labeling. SPION are indicated by arrowheads in magnified images. Data are representative of N = 2 non-infected monkeys, 14 days post i.c. SPION inoculation

    Article Snippet: IHC was performed as previously described, using antibodies targeting CD163 (1:250, Leica (Deer Park, IL)), CD68 (1:100, DAKO (Carpinteria, CA)), CD206 (1:1000, R&D Systems (Minneapolis, MN)), and IBA1 macrophages (1:100, Wako (Osaka, Japan)) [ , ].

    Techniques: Staining, Immunohistochemistry, Labeling, Infection